LOCUS       sequence-93190-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression vector encoding bicistronic EGFP and Puromycin
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Giannou AD, Marazioti A, Spella M, Kanellakis NI, Apostolopoulou H, 
            Psallidas I, Prijovich ZM, Vreka M, Zazara DE, Lilis I, 
            Papaleonidopoulos V, Kairi CA, Patmanidi AL, Giopanou I, Spiropoulou
            N, Harokopos V, Aidinis V, Spyratos D, Teliousi S, Papadaki H, 
            Taraviras S, Snyder LA, Eickelberg O, Kardamakis D, Iwakura Y, 
            Feyerabend TB, Rodewald HR, Kalomenidis I, Blackwell TS, Agalioti T,
            Stathopoulos GT
  TITLE     Mast cells mediate malignant pleural effusion formation.
  JOURNAL   J Clin Invest. 2015 Jun;125(6):2317-34. doi: 10.1172/JCI79840. Epub 
            2015 Apr 27.
  PUBMED    25915587
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI79840"; 
            journalName: "J Clin Invest"; date: "2015-06"; volume: "125"; issue:
            "6"; pages: "2317-34"
COMMENT     Sequence Label: retro-gfp-puro vector
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..656)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(140..160)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     637..656
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(830..849)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gatgcatggg gtcgtgcgct cctttcggtc gggcgctgcg ggtcgtgggg cgggcgtcag
       61 gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg
      121 gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg
      181 aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac ggcgctgccc
      241 agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc
      301 ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag ccgggaaccg
      361 ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc gacgctctcc
      421 ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg
      481 agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat gtggcggtcc
      541 ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg
      601 gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggtt
      661 gtggccatat tatcatcgtg tttttcaaag gaaaaccacg tccccgtggt tcggggggcc
      721 tagacgtttt tttaacctcg actaaacaca tgtaaagcat gtgcaccgag gccccagatc
      781 agatcccata caatggggta ccttctgggc atccttcagc cccttgttga atacgcttga
      841 ggagagccat ttgactcttt ccacaactat ccaactcaca acgtggcact ggggttgtgc
      901 cgccttt
//
