LOCUS       sequence-93195-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKO2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..80
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..758
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 agtgaaccgt cagatccgct agcgccacca tgggcgtggc cgacttgatc aagaagttcg
       61 agtccatctc caaggaggag ggggatccac cggtcgccac catggtgagt gtgattaaac
      121 cagagatgaa gatgaggtac tacatggacg gctccgtcaa tgggcatgag ttcacaattg
      181 aaggtgaagg cacaggcaga ccttacgagg gacatcaaga gatgacacta cgcgtcacaa
      241 tggccgaggg cgggccaatg cctttcgcgt ttgacttagt gtcacacgtg ttctgttacg
      301 gccacagagt atttactaaa tatccagaag agataccaga ctatttcaaa caagcatttc
      361 ctgaaggcct gtcatgggaa aggtcgttgg agttcgaaga tggtgggtcc gcttcagtca
      421 gtgcgcatat aagccttaga ggaaacacct tctaccacaa atccaaattt actggggtta
      481 actttcctgc cgatggtcct atcatgcaaa accaaagtgt tgattgggag ccatcaaccg
      541 agaaaattac tgccagcgac ggagttctga agggtgatgt tacgatgtac ctaaaacttg
      601 aaggaggcgg caatcacaaa tgccaaatga agactactta caaggcggca aaagagattc
      661 ttgaaatgcc aggagaccat tacatcggcc atcgcctcgt caggaaaacc gaaggcaaca
      721 ttactgagca ggtagaagat gcagtagctc atagctaagc ggccgcgact ctagatcata
      781 atcagccata ccacatttgt agaggtt
//
