LOCUS       sequence-93199-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Dendra2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..76
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             98..790
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 aaccgtcaga tccgctagcg ccaccatggg cgtggccgac ttgatcaaga agttcgagtc
       61 catctccaag gaggaggggg atccaccggt cgccaccatg aacaccccgg gaattaacct
      121 gatcaaggag gacatgcgcg tgaaggtgca catggagggc aacgtgaacg gccacgcctt
      181 cgtgatcgag ggcgagggca agggcaagcc ctacgagggc acccagaccg ccaacctgac
      241 cgtgaaggag ggcgcccccc tgcccttcag ctacgacatc ctgaccaccg ccgtgcacta
      301 cggcaaccgg gtgttcacca agtaccccga ggacatcccc gactacttca agcagagctt
      361 ccccgagggc tacagctggg agcgcaccat gaccttcgag gacaagggca tctgcaccat
      421 ccgcagcgac atcagcctgg agggcgactg cttcttccag aacgtgcgct tcaagggcac
      481 caacttcccc cccaacggcc ccgtgatgca gaagaagacc ctgaagtggg agcccagcac
      541 cgagaagctg cacgtgcgcg acggcctgct ggtgggcaac atcaacatgg ccctgctgct
      601 ggagggcggc ggccactacc tgtgcgactt caagaccacc tacaaggcca agaaggtggt
      661 gcagctgccc gacgcccact tcgtggacca ccgcatcgag atcctgggca acgacagcga
      721 ctacaacaag gtgaagctgt acgagcacgc cgtggcccgc tacagccccc tgcccagcca
      781 ggtgtggtaa gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
      841 tacttgcttt aaa
//
