LOCUS       sequence-93204-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Nucleus-7
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..60
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             64..84
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             88..108
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    238..359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(275..294)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     329..348
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(366..821)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(503..524)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     715..734
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gacgagctgt acaagtccgg actcagatct cgagctgatc caaaaaagaa gagaaaggta
       61 gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtagg atccaccgga
      121 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
      181 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
      241 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
      301 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
      361 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
      421 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      481 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      541 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      601 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      661 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      721 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      781 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca ctt
//
