LOCUS       sequence-93209-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, 
            Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, 
            Chudakov DM
  TITLE     Bright monomeric red fluorescent protein with an extended 
            fluorescence lifetime.
  JOURNAL   Nat Methods. 2007 Jul;4(7):555-7. Epub 2007 Jun 17.
  PUBMED    17572680
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2007-07"; volume: "4(7)"; pages: "555-7. Epub 2007
            Jun 17"
COMMENT     Sequence Label: mTagRFP-N1
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..818
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg tctaagggcg
      121 aagagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc
      181 accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
      241 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct
      301 tcatgtacgg cagcagaacc ttcatcaacc acacccaggg catccccgac ttctttaagc
      361 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
      421 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca
      481 gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
      541 ccaacaccga gatgctgtac cccgctgacg gcggcctgga aggcagaagc gacatggccc
      601 tgaagctcgt gggcgggggc cacctgatct gcaacttcaa gaccacatac agatccaaga
      661 aacccgctaa gaacctcaag atgcccggcg tctactatgt ggaccacaga ctggaaagaa
      721 tcaaggaggc cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact
      781 gcgacctccc tagcaaactg gggcacaaac ttaattgagc ggccgcgact ctagatcata
      841 atcagccata ccacatttgt agaggtt
//
