LOCUS       sequence-93211-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mTagRFP-T-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..832
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtcgccacc atggtgtcta agggcgaaga
      121 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca
      181 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
      241 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat
      301 gtacggcagc agaaccttca tcaaccacac ccagggcatc cccgatttct ttaagcagtc
      361 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
      421 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
      481 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccaa
      541 caccgagatg ctgtaccccg ctgacggcgg cctggaaggc agaaccgaca tggccctgaa
      601 gctcgtgggc gggggccacc tgatctgcaa cttcaagacc acatacagat ccaagaaacc
      661 cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac cacagactgg aaagaatcaa
      721 ggaggccgac aaagagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga
      781 cctccctagc aaactggggc acaaacttaa tggcatggac gagctgtaca agtaagcggc
      841 cgcgactcta gatcataatc agc
//
