LOCUS       sequence-93212-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: CYPet-N1
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(105..107,108..110,111..824)
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(411..430)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggag
      121 aggaactgtt cggcggcatc gtgcccatcc tggtggagct ggagggcgac gtgaacggcc
      181 acaagttcag cgtgagcggc gagggcgagg gcgacgccac ctacggcaag ctgaccctga
      241 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctggtg accaccctga
      301 cctggggcgt gcagtgcttc agccggtacc ccgaccacat gaagcagcac gacttcttca
      361 agagcgtgat gcccgagggc tacgtgcagg agcggaccat cttcttcaag gacgacggca
      421 actacaagac ccgggccgag gtgaagttcg agggcgacac cctggtgaac cggatcgagc
      481 tgaagggcat cgacttcaag gaggacggca acatcctggg ccacaagctg gagtacaact
      541 acatcagcca caacgtgtac atcaccgccg acaagcagaa gaacggcatc aaggccaact
      601 tcaaggcccg gcacaacatc accgacggca gcgtgcagct ggccgaccac taccagcaga
      661 acacccccat cggcgacggc cccgtgatcc tgcccgacaa ccactacctg agcacccaga
      721 gcgccctgag caaggacccc aacgagaagc gggaccacat ggtgctgctg gagttcgtga
      781 ccgccgccgg catcacccac ggcatggacg aactgtacaa ataaagcggc cgcgactcta
      841 gatcataatc agccatacca catttgtaga ggtt
//
