LOCUS       sequence-93215-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-NEEP21-N-18
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             506..517
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             527..538
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      619..628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(657..756)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(686..706)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccttgctcac catggtggcg accggtggat ccgcgctacc actggctgcg cttgctccac
       61 cgctgctggc tgacttctct gcagcttcgg tctcctgttc cgacagcttc tcttctgaca
      121 gaactgacat ccatggcgac acggagcggg tgatgctctg cttggccacg ttgtagtggt
      181 ttatgacagt gtaaaatttc tcccgggcac tggagtcttg ctccgtgtag tagctctcca
      241 agccttctgg gatgcactgg gtgttcttca agacaaaccc accagggcag gcgcggtcat
      301 acttgtacac tttgtagaca accaggaaga cgacacaggt gaggaaggcc agggcaaaga
      361 ggaccagcac ggagacctta aacctctcgg tgacaccctc ggtgatgctg acggtgaact
      421 cggctatctt gggaggacgt gcttttcctt ttttgcgatc aggttcatat tcagtcttag
      481 ttttcaccac gaccttatct gggggtggga actgcagctg gttgacatcg aggggcgtca
      541 tcaaaggaat ggtgtcgaag ccatcctcca gcagtggctg cttggtgccc ttctctgcga
      601 aattattccc caacttcacc atggtggcgc tagcggatct gacggttcac taaaccagct
      661 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      721 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      781 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
      841 tactgccaaa
//
