LOCUS       sequence-93220-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mCerulean-Nucleus-12
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacg ccatcagcga
      481 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatcctccg gactcagatc
      781 tcgagctgat ccaaa
//
