LOCUS       sequence-93227-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 450, Emission: 495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: iLOV-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..75
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     polyA_signal    554..675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(591..610)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     645..664
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 accgtcagat ccgctagcgc caccatgggc gtggccgact tgatcaagaa gttcgagtcc
       61 atctccaagg aggaggggga tccaccggtc gccaccatga tcgagaagaa cttcgtgatc
      121 accgacccca gactgcccga caaccccatc atcttcgcca gcgacggctt cctggagctg
      181 accgagtaca gcagagagga gatcctgggc agaaacgcca gattcctgca gggccccgag
      241 accgaccagg ccaccgtgca gaagatcaga gacgccatca gagaccagag agagaccacc
      301 gtgcagctga tcaactacac caagagcggc aagaagttct ggaacctgct gcacctgcag
      361 cccgtgagag accagaaggg cgagctgcag tacttcatcg gcgtgcagct ggacggcagc
      421 gaccacgtgt aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
      481 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
      541 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      601 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      661 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
      721 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaa
//
