LOCUS       sequence-93229-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for knockdown of human PIK3CD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Bajikar SS, Fuchs C, Roller A, Theis FJ, Janes KA
  TITLE     Parameterizing cell-to-cell regulatory heterogeneities via 
            stochastic transcriptional profiles.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):E626-35. doi: 
            10.1073/pnas.1311647111. Epub 2014 Jan 21.
  PUBMED    24449900
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1311647111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-4- 4"; volume: "111"; issue: "5"; pages: "E626-35"
COMMENT     Sequence Label: pLKO.1 shPIK3CD.v2 puro
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     126..145
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    297..414
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa
       61 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
      121 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
      181 tcttgtggaa aggacgaaac accgggaccc agaagtgaac gactttctgc agaaagttca
      241 cttctgggtc tttttgaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt
      301 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      361 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      421 tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct
      481 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
      541 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
      601 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
      661 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
      721 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
      781 tgtggccaat agcggctgct cagcagggcg cgccgagagc a
//
