LOCUS       sequence-93230-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses diphtheria toxin A subunit in Cre positive cells, and 
            mCherry in all the infected cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Wu Z, Autry AE, Bergan JF, Watabe-Uchida M, Dulac CG
  TITLE     Galanin neurons in the medial preoptic area govern parental 
            behaviour.
  JOURNAL   Nature. 2014 May 15;509(7500):325-30. doi: 10.1038/nature13307.
  PUBMED    24828191
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2014-05-15- 15"; volume: "509"; issue: "7500"; 
            pages: "325-30"
COMMENT     Sequence Label: pAAV-mCherry-flex-dtA
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..806
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(241..259)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     509..528
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttccatttca ggtgtcgtga ggtaccggat cctctagagt caattcatta cctcgtatag
       61 gatactttat acgaagttat gccgccacca tgggtggggt gagcaagggc gaggaggata
      121 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      181 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      241 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      301 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      361 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
      421 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
      481 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
      541 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
      601 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
      661 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
      721 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
      781 gcggcatgga cgagctgtac aagtaataac ctcgtatagc atacattata cgaagtaata
      841 gatcttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta
      901 aggttccttc acaaa
//
