LOCUS       sequence-93234-e         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Magnetic cell sorting of ES cell-derived astrocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Bryson JB, Machado CB, Crossley M, Stevenson D, Bros-Facer V, 
            Burrone J, Greensmith L, Lieberam I
  TITLE     Optical control of muscle function by transplantation of stem 
            cell-derived motor neurons in mice.
  JOURNAL   Science. 2014 Apr 4;344(6179):94-7. doi: 10.1126/science.1248523.
  PUBMED    24700859
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-04-4- 4"; volume: "344"; issue: 
            "6179"; pages: "94-7"
COMMENT     Sequence Label: tol2-GFAP::CD14
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          37..266
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     249..268
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(392..409)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    398..622
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggcgcgcctc gctgtctgcg agggccagct gttggggtga gtactccctc tcaaaagcgg
       61 gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct
      121 ggcccgcggt gatgcctttg agggtggccg cgtccatctg gtcagaaaag acaatctttt
      181 tgttgtcaag cttgaggtgt ggcaggcttg agatctggcc atacacttga gtgacaatga
      241 catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc agcccaagcg
      301 gatcacccgg accggtcctg cagggtttaa acggccggcc attgactagt agtgccatgg
      361 gggcccgcta gccctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
      421 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      481 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
      541 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
      601 ggggatgcgg tgggctctat ggcttctgag gaaagaacca gctggggctc gagtctaga
//
