LOCUS       sequence-93235-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 610, Emission: 652.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mMaroon-N1
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..836
                     /codon_start=1
                     /product="bright, rapidly maturing, monomeric far-red 
                     fluorescent protein (Bajar et al., 2016)"
                     /label=mMaroon1
                     /translation="MVSKGEELIKENMHTKLYLTGTVNNHYFECTAEGEGKPYEGTQTN
                     RIKVVRGGPLPFAFDILAPCFMYGSKTFINHPPDIPDYFKQSFPEGFTWERTTVYEDGG
                     TLTATQDTSLQDGCLIYNVQVRGENFPSNGPVMQKKTLGWEASTETLYPADGSLEGRLD
                     WALKLVGGGHLHCRLETTYRSKKPAKNLKMPGVYFIDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg
      121 aggagctgat caaggagaac atgcacacca agctgtacct gacaggcacc gtgaacaacc
      181 actacttcga atgcaccgcc gaaggggagg gcaagcccta cgagggcacc cagaccaaca
      241 ggatcaaggt ggtgcgcgga ggccccctgc cgttcgcatt cgacatcctg gccccctgct
      301 ttatgtacgg gagcaagacc ttcattaacc atccacctga cattcccgat tattttaagc
      361 agtccttccc tgagggcttc acatgggaga gaaccaccgt ttacgaagac gggggcacac
      421 tcactgctac acaggacacc agcctccagg acggctgcct gatctacaac gtccaagtca
      481 gaggggagaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
      541 ccagtaccga gaccctgtac cccgctgacg gcagcctgga aggcagactg gactgggccc
      601 tgaagctcgt gggcgggggc cacctgcact gcagactgga aaccacatac agatccaaga
      661 aacccgcaaa gaacctcaag atgcccggcg tctattttat tgacaggaga ctggaaagaa
      721 tcaaggaggc cgacaatgag acctacgtcg agcagcacga ggtggctgtg gccagatact
      781 gcgacctccc tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaag
//
