LOCUS       sequence-93236-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for knockdown of human PIK3CD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Bajikar SS, Fuchs C, Roller A, Theis FJ, Janes KA
  TITLE     Parameterizing cell-to-cell regulatory heterogeneities via 
            stochastic transcriptional profiles.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):E626-35. doi: 
            10.1073/pnas.1311647111. Epub 2014 Jan 21.
  PUBMED    24449900
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1311647111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-4- 4"; volume: "111"; issue: "5"; pages: "E626-35"
COMMENT     Sequence Label: pLKO.1 shPIK3CD.v2 puro
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(51..299)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(109..128)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(279..299)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(597..641)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aaagacccag aagtgaactt tctgcagaaa gtcgttcact tctgggtccc ggtgtttcgt
       61 cctttccaca agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata
      121 tgatagtcca ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt
      181 ctacgtcacg tattttgtac taatatcttt gtgtttacag tcaaattaat tccaattatc
      241 tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctcg
      301 gtgaaggggg cggccgctcg aggctagtct cgtgatcgat accgtcgaga tccgttcact
      361 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
      421 tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
      481 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
      541 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
      601 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
      661 tcttcaatta aggagt
//
