LOCUS       sequence-93244-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Dronpa-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..98
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..780
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagtgtgatt
      121 aaaccagaca tgaagatcaa gctgcgtatg gaaggcgctg taaatggaca cccgttcgcg
      181 attgaaggag ttggccttgg gaagcctttc gagggaaaac agagtatgga ccttaaagtc
      241 aaagaaggcg gacctctgcc tttcgcctat gacatcttga caactgtgtt ctgttacggc
      301 aacagggtat tcgccaaata cccagaaaat atagtagact atttcaagca gtcgtttcct
      361 gagggctact cttgggaacg aagcatgaat tacgaagacg ggggcatttg taacgcgaca
      421 aacgacataa ccctggatgg tgactgttat atctatgaaa ttcgatttga tggtgtgaac
      481 tttcctgcca atggtccagt tatgcagaag aggactgtga aatgggagcc atccactgag
      541 aaattgtatg tgcgtgatgg agtgctgaag ggtgatgtta acatggctct gtcgcttgaa
      601 ggaggtggcc attaccgatg tgacttcaaa actacttata aagctaagaa ggttgtccag
      661 ttgccagact atcactttgt ggaccaccac attgagatta aaagccacga caaagattac
      721 agtaatgtta atctgcatga gcacgccgaa gcgcattctg agctgccgag gcaggccaag
      781 tccggatcca agctgtagcg gccgcgactc tagatcataa tcagccatac cacatttgta
      841 gaggtt
//
