LOCUS       sequence-93251-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites
            removed and T821 restored.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Narasimha AM, Kaulich M, Shapiro GS, Choi YJ, Sicinski P, Dowdy SF
  TITLE     Cyclin D activates the Rb tumor suppressor by mono-phosphorylation.
  JOURNAL   Elife. 2014 May 29:e02872. doi: 10.7554/eLife.02872.
  PUBMED    24876129
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            May 29:e02872. doi: 10.7554/eLife.02872."
COMMENT     Sequence Label: pCMV  HA hRB delta CDK + T821
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          129..225
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             289..315
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
       61 gaccgatcca gcctccggac tctagaggat ccggtactag aggaactgaa aaaccagaaa
      121 gttaactggt aagtttagtc tttttgtctt ttatttcagg tcccggatcc ggtggtggtg
      181 caaatcaaag aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg
      241 ttacttctgc tctaaaagct gcggaattgt acccgcgggc ccaccatgta cccatacgat
      301 gttccagatt acgctcttat ggccatggag gcccgaattc ggtcgacccc gcccaaagcc
      361 ccccgaaaaa cggccgccac cgccgccgct gccgccgcgg aacccccggc accgccgccg
      421 ccgccccctc ctgaggagga cccagagcag gacagcggcc cggaggacct gcctctcgtc
      481 aggcttgagt ttgaagaaac agaagaacct gattttactg cattatgtca gaaattaaag
      541 ataccagatc atgtcagaga gagagcttgg ttaacttggg agaaagtttc atctgtggat
      601 ggagtattgg gaggttatat tcaaaagaaa aaggaactgt ggggaatctg tatctttatt
      661 gcagcagttg acctagatga gatgtcgttc acttttactg agctacagaa aaacatagaa
      721 atc
//
