LOCUS       sequence-93256-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-N1
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..835
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga
      121 gctgatcaag gagaacatgc acatgaagct gtacatggaa ggcaccgtga acaaccacca
      181 cttcaagtgc accaccgaag gggagggcaa gccctacgag ggcacccaga cccagaggat
      241 taaggtggtg gagggaggcc ccctgccgtt cgcattcgac atcctggcca cctgctttat
      301 gtacgggagc aagaccttca tcaaccacac ccagggcatc cccgatttct ttaagcagtc
      361 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgcttac
      421 cgttacccag gacaccagcc tccaggacgg ctgcttgatc tacaacgtca agctcagagg
      481 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccac
      541 caccgagacc ctgtaccccg ctgacggcgg cctggaaggc agatgcgaca tggccctgaa
      601 gctcgtgggc gggggccacc tgcactgcaa cctgaagacc acatacagat ccaagaaacc
      661 cgctaagaac ctcaagatgc ccggcgtcta ctttgtggac cgcagactgg aaagaatcaa
      721 ggaggccgac aatgagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga
      781 cctccctagc aaactggggc acaaacttaa tggcatggac gagctgtaca agtaagcggc
      841 cgcgactcta gatcataatc agccatacca catttgtaga ggtt
//
