LOCUS       sequence-93258-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for genomic targeting of a Tetracycline-inducible Cas9 
            cassette to the human AAVS1/PPP1R12C locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D
  TITLE     An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome 
            Editing in Human Pluripotent Stem Cells.
  JOURNAL   Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018.
  PUBMED    24931489
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018."
COMMENT     Sequence Label: Puro-Cas9 donor
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    188..717
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 aattgtaata cgactcacta tagggcgaat tgaatttagc ggccgcgaat tcgcccttag
       61 agcagagcca ggaacccctg tagggaaggg gcaggagagc caggggcatg agatggtgga
      121 cgaggaaggg ggacagggaa gcctgagcgc ctctcctggg cttgccaagg actcaaaccc
      181 agaagcccag agcagggcct tagggaagcg ggaccctgct ctgggcggag gaatatgtcc
      241 cagatagcac tggggactct ttaaggaaag aaggatggag aaagagaaag ggagtagagg
      301 cggccacgac ctggtgaaca cctaggacgc accattctca caaagggagt tttccacacg
      361 gacacccccc tcctcaccac agccctgcca ggacggggct ggctactggc cttatctcac
      421 aggtaaaact gacgcacgga ggaacaatat aaattgggga ctagaaaggt gaagagccaa
      481 agttagaact caggaccaac ttattctgat tttgtttttc caaactgctt ctcctcttgg
      541 gaagtgtaag gaagctgcag caccaggatc agtgaaacgc accagacagc cgcgtcagag
      601 cagctcaggt tctgggagag ggtagcgcag ggtggccact gagaaccggg caggtcacgc
      661 atccccccct tccctcccac cccctgccaa gctctccctc ccaggatcct ctctggctcc
      721 atcgtaagca aaccttagag gttctggcaa ggagagagat ggctccagga aatgggggtg
      781 tgtcaccaga taaggaatct gcctaacagg aggtgggg
//
