LOCUS       sequence-9326-ex        1148 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Sagermann M, Baase WA, Matthews BW
  TITLE     Sequential reorganization of beta-sheet topology by insertion of a 
            single strand.
  JOURNAL   Protein Sci. 2006 May . 15(5):1085-92.
  PUBMED    16597830
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2006 May . 15(5):1085-92."
COMMENT     Sequence Label: T4 Lysozyme L30C
FEATURES             Location/Qualifiers
     source          1..1148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    742..758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(766..772,773..788,789..794))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    837..853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(861..867,868..883,884..888))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 aattgcaaaa agcccgctca ttaggcgggc tcagatctgc tcatgtttga cagcttatca
       61 tcgataagct tcggcaataa attcttgata tgttttcata tgtttccttt aaatatttta
      121 attagtaatt gtctattcaa gtaattgtga atatactatc acaattccaa gagaaagtaa
      181 acagctttat agatttttat acgcgtccca agtgccagtt ctaaacgttg taatgactcg
      241 ttttgcgcga ttaggtgttt gattatacca tctactttta gctaagttaa ctgctgcttc
      301 atcccagcgt ttttgttgaa gcatacgtaa agagttagta aatcctgcca caccggtttc
      361 tcccatttgg aaaaccatat taatcaatgc agcgcgacga accgcatcaa gagaatcata
      421 aaccggtttt aatttagcat ttctcagaat tccgcgaaca gcagcatcaa catcctgatt
      481 aaagagtttt tcagcctcgt cttttgtaat tacaccatta gtattacgcc caatagcttt
      541 atctaattca gatttagcag cattaagtga tggacttttt gtaagcaaat gaccgatgcc
      601 aatagtgtag ccaataccga tggtataata gccttctgtg tctttataga ttttaagtct
      661 aagaccttca tctatacgta acatttcaaa tatattcata atacctccta agcatcgatg
      721 gatcctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacatta tacgagccga
      781 tgattaattg tcaacagggg gatggggagt aagctgatcc tgtttcctgt gtgaaattgt
      841 tatccgctca caattccaca cattatacga gccgatgatt aattgtcaca gggatgggag
      901 tagctcatcg atgatcgtcg ctttcctctt ttcttttttt aaaaattgtt atccgcccaa
      961 caattccaac acatttatac ggagccggaa gcattaagtg taagctgggg gtgctatgag
     1021 tgagctactt acatatgcgt gcgctcactg ccgcttcagt ccgactgtcc tgcagacaca
     1081 tcgatgtgcc acttccgatt gcatatagcc ggagagtcat cagggtactg gtaaccgtac
     1141 gttagcag
//
