LOCUS       sequence-93271-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for genomic targeting of a Tetracycline-inducible Cas9 
            cassette to the human AAVS1/PPP1R12C locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D
  TITLE     An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome 
            Editing in Human Pluripotent Stem Cells.
  JOURNAL   Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018.
  PUBMED    24931489
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018."
COMMENT     Sequence Label: Puro-Cas9 donor
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    260..761
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 tgaccatgat tacgccaagc tcagaattaa ccctcactaa agggactagt cctgcaggtt
       61 taaacgaatt cgccctttgc tttctctgac cagcattctc tcccctgggc ctgtgccgct
      121 ttctgtctgc agcttgtggc ctgggtcacc tctacggctg gcccagatcc ttccctgccg
      181 cctccttcag gttccgtctt cctccactcc ctcttcccct tgctctctgc tgtgttgctg
      241 cccaaggatg ctctttccgg agcacttcct tctcggcgct gcaccacgtg atgtcctctg
      301 agcggatcct ccccgtgtct gggtcctctc cgggcatctc tcctccctca cccaacccca
      361 tgccgtcttc actcgctggg ttcccttttc cttctccttc tggggcctgt gccatctctc
      421 gtttcttagg atggccttct ccgacggatg tctcccttgc gtcccgcctc cccttcttgt
      481 aggcctgcat catcaccgtt tttctggaca accccaaagt accccgtctc cctggcttta
      541 gccacctctc catcctcttg ctttctttgc ctggacaccc cgttctcctg tggattcggg
      601 tcacctctca ctcctttcat ttgggcagct cccctacccc ccttacctct ctagtctgtg
      661 ctagctcttc cagccccctg tcatggcatc ttccaggggt ccgagagctc agctagtctt
      721 cttcctccaa cccgggcccc tatgtccact tcaggacagc atgtttgctg cctccaggga
      781 tcctgtgtcc ccgagctggg accaccttat attcccaggg ccggttaatg tggctctggt
      841 tctgggtact tttatctgtc ccctccaccc cacagtgggg
//
