LOCUS       sequence-93272-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for genomic targeting of a Tetracycline-inducible Cas9 
            cassette to the human AAVS1/PPP1R12C locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D
  TITLE     An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome 
            Editing in Human Pluripotent Stem Cells.
  JOURNAL   Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018.
  PUBMED    24931489
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018."
COMMENT     Sequence Label: Puro-Cas9 donor
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..36
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             70..135
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             142..162
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aattccgatc aacaagtttg tacaaaaaag caggctccgc ggttttcagg ttggaccggt
       61 gccaccatgg actataagga ccacgacgga gactacaagg atcatgatat tgattacaaa
      121 gacgatgacg ataagatggc cccaaagaag aagcggaagg tcggtatcca cggagtccca
      181 gcagccgaca agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc
      241 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac
      301 cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc
      361 gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc
      421 tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac
      481 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc
      541 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga
      601 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
      661 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc
      721 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac
      781 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc
      841 agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga
      901 aacctgattg ccctgagcct gggcctgacc cccaacttca
//
