LOCUS       sequence-93273-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for genomic targeting of a Tetracycline-inducible Cas9 
            cassette to the human AAVS1/PPP1R12C locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D
  TITLE     An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome 
            Editing in Human Pluripotent Stem Cells.
  JOURNAL   Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018.
  PUBMED    24931489
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018."
COMMENT     Sequence Label: Puro-Cas9 donor
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctcaggccg cctctctcgg ccttggtcag attgtcgaac tttctctggg taatcagctt
       61 ggcgttcagc agctgccgcc agtagttctt catcttcttc acgacctctt cggagggcac
      121 gttgtcgctc ttgccccggt tcttgtcgct tctggtcagc accttgttgt cgatggagtc
      181 gtccttcaga aagctctgag gcacgatatg gtccacatcg tagtcggaca gccggttgat
      241 gtccagttcc tggtccacgt acatatcccg cccattctgc aggtagtaca ggtacagctt
      301 ctcgttctgc agctgggtgt tttccacggg gtgttctttc aggatctggc tgcccagctc
      361 tttgatgccc tcttcgatcc gcttcattct ctcgcggctg ttcttctgtc ccttctgggt
      421 ggtctggttc tctctggcca tttcgatcac gatgttctcg ggcttgtgcc ggcccatcac
      481 tttcacgagc tcgtccacca ccttcactgt ctgcaggatg cccttcttaa tggcggggct
      541 gccggccaga ttggcaatgt gctcgtgcag gctatcgccc tggccggaca cctgggcttt
      601 ctggatgtcc tctttaaagg tcaggctgtc gtcgtggatc agctgcatga agtttctgtt
      661 ggcgaagccg tcggacttca ggaaatccag gattgtcttg ccggactgct tgtcccggat
      721 gccgttgatc agcttccggc tcagcctgcc ccagccggtg tatctccgcc gcttcagctg
      781 cttcatcact ttgtcgtcga acaggtgggc ataggttttc agccgttcct cgatcatctc
      841 tctgtcctca a
//
