LOCUS       sequence-93319-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites
            removed and S249 restored .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Narasimha AM, Kaulich M, Shapiro GS, Choi YJ, Sicinski P, Dowdy SF
  TITLE     Cyclin D activates the Rb tumor suppressor by mono-phosphorylation.
  JOURNAL   Elife. 2014 May 29:e02872. doi: 10.7554/eLife.02872.
  PUBMED    24876129
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            May 29:e02872. doi: 10.7554/eLife.02872."
COMMENT     Sequence Label: pCMV  HA hRB delta CDK + S249
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          132..228
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             292..318
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      121 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      181 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      241 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
      301 gatgttccag attacgctct tatggccatg gaggcccgaa ttcggtcgac cccgcccaaa
      361 gccccccgaa aaacggccgc caccgccgcc gctgccgccg cggaaccccc ggcaccgccg
      421 ccgccgcccc ctcctgagga ggacccagag caggacagcg gcccggagga cctgcctctc
      481 gtcaggcttg agtttgaaga aacagaagaa cctgatttta ctgcattatg tcagaaatta
      541 aagataccag atcatgtcag agagagagct tggttaactt gggagaaagt ttcatctgtg
      601 gatggagtat tgggaggtta tattcaaaag aaaaaggaac tgtggggaat ctgtatcttt
      661 attgcagcag ttgacctaga tgagatgtcg ttcactttta ctgagctaca gaa
//
