LOCUS       sequence-93322-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 472, Emission: 495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A
  TITLE     Cyan-emitting and orange-emitting fluorescent proteins as a 
            donor/acceptor pair for fluorescence resonance energy transfer.
  JOURNAL   Biochem J. 2004 Jul 1. 381(Pt 1):307-12.
  PUBMED    15065984
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2004 Jul 1. 381(Pt 1):307-12."
COMMENT     Sequence Label: MiCy-C1
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..735
                     /codon_start=1
                     /product="dimeric Midoriishi-Cyan fluorescent protein"
                     /label=MiCy
                     /translation="MVSYSKQGIAQEMRTKYRMEGSVNGHEFTIEGVGTGNPYEGKQMS
                     ELVIIKPKGKPLPFSFDILSTAFQYGNRCFTKYPADMPDYFKQAFPDGMSYERSFLFED
                     GGVATASWSIRLEGNCFIHNSIYHGVNFPADGPVMKKQTIGWDKSFEKMSVAKEVLRGD
                     VTQFLLLEGGGYQRCRFHSTYKTEKPVAMPPSHVVEHQIVRTDLGQTAKGFKVKLEEHA
                     EAHVNPLKVK"
     misc_feature    736..801
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgtctta ttcaaagcaa
       61 ggcatcgcac aagaaatgcg gacgaaatac cgtatggaag gcagtgtcaa tggccatgag
      121 ttcacgatcg aaggtgtagg aactggaaac ccttacgaag ggaaacagat gtccgaatta
      181 gtgatcatca agcctaaggg aaaacccctt ccattctcct ttgacatact gtcaacagcc
      241 tttcaatatg gaaacagatg cttcacaaag taccctgccg acatgcctga ctatttcaag
      301 caagcattcc cagatggaat gtcatatgaa aggtcatttc tatttgagga tggaggagtt
      361 gctacagcca gctggagcat tcgtctcgaa ggaaattgct tcatccacaa ttccatctat
      421 catggcgtaa actttcccgc tgatggaccc gtaatgaaga agcagacaat tggctgggat
      481 aagtccttcg aaaaaatgag tgtggctaaa gaggtgctaa gaggtgatgt gactcagttt
      541 cttctgctcg aaggaggtgg ttaccagaga tgccggtttc actccactta caaaacggag
      601 aagccagtcg caatgccccc gagtcatgtc gtagaacatc aaattgtgag gaccgacctt
      661 ggccaaactg caaaaggctt caaggtcaag ctggaagaac atgctgaggc tcatgttaac
      721 cctttgaagg ttaaatccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
      781 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca
      841 tt
//
