LOCUS       sequence-93325-e         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: EBFP2-N1
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(146..167)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga
      121 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      181 gttcagcgtg aggggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt
      241 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgagcca
      301 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      361 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaccta
      421 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      481 gggcgtcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa
      541 cagccacaac atctatatca tggccgtcaa gca
//
