LOCUS       sequence-93331-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: CYPet-C1
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(39..41,42..44,45..755)
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(345..364)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggagaggaac
       61 tgttcggcgg catcgtgccc atcctggtgg agctggaggg cgacgtgaac ggccacaagt
      121 tcagcgtgag cggcgagggc gagggcgacg ccacctacgg caagctgacc ctgaagttca
      181 tctgcaccac cggcaagctg cccgtgccct ggcccaccct ggtgaccacc ctgacctggg
      241 gcgtgcagtg cttcagccgg taccccgacc acatgaagca gcacgacttc ttcaagagcg
      301 tgatgcccga gggctacgtg caggagcgga ccatcttctt caaggacgac ggcaactaca
      361 agacccgggc cgaggtgaag ttcgagggcg acaccctggt gaaccggatc gagctgaagg
      421 gcatcgactt caaggaggac ggcaacatcc tgggccacaa gctggagtac aactacatca
      481 gccacaacgt gtacatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaagg
      541 cccggcacaa catcaccgac ggcagcgtgc agctggccga ccactaccag cagaacaccc
      601 ccatcggcga cggccccgtg atcctgcccg acaaccacta cctgagcacc cagagcgccc
      661 tgagcaagga ccccaacgag aagcgggacc acatggtgct gctggagttc gtgaccgccg
      721 ccggcatcac ccacggcatg gacgaactgt acaaatccgg actcagatct cgagctcaag
      781 cttcgaattc tgcagtcgac ggtaccgcg
//
