LOCUS       sequence-93336-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Rego EH, Shao L, Macklin JJ, Winoto L, Johansson GA, Kamps-Hughes N,
            Davidson MW, Gustafsson MG
  TITLE     Nonlinear structured-illumination microscopy with a photoswitchable 
            protein reveals cellular structures at 50-nm resolution.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 17;109(3):E135-43. doi: 
            10.1073/pnas.1107547108. Epub 2011 Dec 12.
  PUBMED    22160683
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1107547108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-01-17- 17"; volume: "109"; issue: "3"; pages: "E135-43"
COMMENT     Sequence Label: Dronpa-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..80
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..779
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 agtgaaccgt cagatccgct agcgccacca tgggcgtggc cgacttgatc aagaagttcg
       61 agtccatctc caaggaggag ggggatccac cggtcgccac catggtgagt gtgattaaac
      121 cagacatgaa gatcaagctg cgtatggaag gcgctgtaaa tggacacccg ttcgcgattg
      181 aaggagttgg ccttgggaag cctttcgagg gaaaacagag tatggacctt aaagtcaaag
      241 aaggcggacc tctgcctttc gcctatgaca tcttgacaac tgtgttctgt tacggcaaca
      301 gggtattcgc caaataccca gaaaatatag tagactattt caagcagtcg tttcctgagg
      361 gctactcttg ggaacgaagc atgaattacg aagacggggg catttgtaac gcgacaaacg
      421 acataaccct ggatggtgac tgttatatct atgaaattcg atttgatggt gtgaactttc
      481 ctgccaatgg tccagttatg cagaagagga ctgtgaaatg ggagccatcc actgagaaat
      541 tgtatgtgcg tgatggagtg ctgaagggtg atgttaacat ggctctgtcg cttgaaggag
      601 gtggccatta ccgatgtgac ttcaaaacta cttataaagc taagaaggtt gtccagttgc
      661 cagactatca ctttgtggac caccacattg agattaaaag ccacgacaaa gattacagta
      721 atgttaatct gcatgagcac gccgaagcgc attctgagct gccgaggcag gccaagtaat
      781 gataggcggc cgcgactcta gatcataatc agccatacca catt
//
