LOCUS       sequence-93337-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Dronpa3-C1
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..714
                     /codon_start=1
                     /product="photoswitchable Dronpa-Green3 fluorescent 
                     protein"
                     /label=Dronpa-Green3
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDINAALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     misc_feature    715..780
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagtgt gattaaacca
       61 gacatgaaga tcaagctgcg tatggaaggc gctgtaaatg gacacccgtt cgcgattgaa
      121 ggagttggcc ttgggaagcc tttcgaggga aaacagagta tggaccttaa agtcaaagaa
      181 ggcggacctc tgcctttcgc ctatgacatc ttgacaactg tgttctgtta cggcaacagg
      241 gtattcgcca aatacccaga aaatatagta gactatttca agcagtcgtt tcctgagggc
      301 tactcttggg aacgaagcat gaattacgaa gacgggggca tttgtaacgc gacaaacgac
      361 ataaccctgg atggtgactg ttatatctat gaaattcgat ttgatggtgt gaactttcct
      421 gccaatggtc cagttatgca gaagaggact gtgaaatggg agccatccac tgagaaattg
      481 tatgtgcgtg atggagtgct gaagggtgat attaacgcgg ctctgtcgct tgaaggaggt
      541 ggccattacc gatgtgactt caaaactact tataaagcta agaaggttgt ccagttgcca
      601 gactatcact ttgtggacca ccacattgag attaaaagcc acgacaaaga ttacagtaat
      661 gttaatctgc atgagcacgc cgaagcgcat tctgagctgc cgaggcaggc caagtccgga
      721 ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc
      781 accggatcta gataactgat cataatcagc cataccacat t
//
