LOCUS       sequence-93351-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 549, Emission:
            565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-pBAD
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..74
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..147
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             160..180
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
       61 ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca tggtatggct
      121 agcatgactg gtggacagca aatgggtcgg gatctgtacg agaacctgta cttccagggc
      181 tcgagcatgg tgagcaaggg cgaggagaat aacatggcca tcatcaagga gttcatgcgc
      241 ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      301 gagggccgcc cctacgaggg ctttcagacc gctaagctga aggtgaccaa gggtggcccc
      361 ctgcccttcg cctgggacat cctgtcccct catttcacct acggctccaa ggcctacgtg
      421 aagcaccccg ccgacatccc cgactacttc aagctgtcct tccccgaggg cttcaagtgg
      481 gagcgcgtga tgaactacga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      541 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      601 cccgtgatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      661 gacggtgccc tgaagggcaa gatcaagatg aggctgaagc tgaaggacgg cggccactac
      721 acctccgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
      781 atcgtcgaca tcaagttgga catcacctcc cacaacgagg actac
//
