LOCUS       sequence-93352-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 568, Emission:
            592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mApple-pBAD
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..88
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..124
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(128..136,137..151)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     580..599
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
       61 ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
      121 gggtcgggat ctgtacgacg atgacgataa ggatccgagc tcgagcatgg tgagcaaggg
      181 cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
      241 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggc
      301 ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      361 cctgtcccct cagttcatgt acggctccaa ggtctacatt aagcacccag ccgacatccc
      421 cgactacttc aagctgtcct tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga
      481 ggacggcggc attattcacg ttaaccagga ctcctccctg caggacggcg tgttcatcta
      541 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
      601 catgggctgg gaggcctccg aggagcggat gtaccccgag gacggcgccc tgaagagcga
      661 gatcaagaag aggctgaagc tgaaggacgg cggccactac gccgccgagg tcaagaccac
      721 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga
      781 catcgtgtcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccga
//
