LOCUS       sequence-93360-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 462, Emission:
            492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Ai HW, Henderson JN, Remington SJ, Campbell RE
  TITLE     Directed evolution of a monomeric, bright and photostable version of
            Clavularia cyan fluorescent protein: structural characterization and
            applications in fluorescence imaging.
  JOURNAL   Biochem J. 2006 Dec 15. 400(3):531-40.
  PUBMED    16859491
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2006 Dec 15. 400(3):531-40."
COMMENT     Sequence Label: mTFP1-pBAD
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..74
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..147
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             160..180
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
       61 ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca tggtatggct
      121 agcatgactg gtggacagca aatgggtcgg gatctgtacg agaacctgta cttccagggc
      181 tcgagcatgg tgagcaaggg cgaggagacc acaatgggcg taatcaagcc cgacatgaag
      241 atcaagctga agatggaggg caacgtgaat ggccacgcct tcgtgatcga gggcgagggc
      301 gagggcaagc cctacgacgg caccaacacc atcaacctgg aggtgaagga gggagccccc
      361 ctgcccttct cctacgacat tctgaccacc gcgttcgcct acggcaacag ggccttcacc
      421 aagtaccccg acgacatccc caactacttc aagcagtcct tccccgaggg ctactcttgg
      481 gagcgcacca tgaccttcga ggacaagggc atcgtgaagg tgaagtccga catctccatg
      541 gaggaggact ccttcatcta cgagatacac ctcaagggcg agaacttccc ccccaacggc
      601 cccgtgatgc agaagaagac caccggctgg gacgcctcca ccgagaggat gtacgtgcgc
      661 gacggcgtgc tgaagggcga cgtcaagcac aagctgctgc tggagggcgg cggccaccac
      721 cgcgttgact tcaaga
//
