LOCUS       sequence-93363-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 551, Emission:
            565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, 
            Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, 
            Masai H, Miyawaki A
  TITLE     Visualizing spatiotemporal dynamics of multicellular cell-cycle 
            progression.
  JOURNAL   Cell. 2008 Feb 8;132(3):487-98. doi: 10.1016/j.cell.2007.12.033.
  PUBMED    18267078
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2007.12"; journalName: "Cell"; date: "2008-02-8- 8";
            volume: "132"; issue: "3"; pages: "487-98"
COMMENT     Sequence Label: mKO2-pBAD
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..74
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..147
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             160..180
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
       61 ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca tggtatggct
      121 agcatgactg gtggacagca aatgggtcgg gatctgtacg agaacctgta cttccagggc
      181 tcgagcatgg tgagtgtgat taaaccagag atgaagatga ggtactacat ggacggctcc
      241 gtcaatgggc atgagttcac aattgaaggt gaaggcacag gcagacctta cgagggacat
      301 caagagatga cactacgcgt cacaatggcc gagggcgggc caatgccttt cgcgtttgac
      361 ttagtgtcac acgtgttctg ttacggccac agagtattta ctaaatatcc agaagagata
      421 ccagactatt tcaaacaagc atttcctgaa ggcctgtcat gggaaaggtc gttggagttc
      481 gaagatggtg ggtccgcttc agtcagtgcg catataagcc ttagaggaaa caccttctac
      541 cacaaatcca aatttactgg ggttaacttt cctgccgatg gtcctatcat gcaaaaccaa
      601 agtgttgatt gggagccatc aaccgagaaa attactgcca gcgacggagt tctgaagggt
      661 gatgttacga tgtacctaaa acttgaagga ggcggcaatc acaaatgcca aatgaagact
      721 acttacaagg cggcaaaaga gattcttgaa atgccaggag accattacat cggccatcgc
      781 ctcgtcagga a
//
