LOCUS       sequence-93365-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 590, Emission:
            649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Wang L, Jackson WC, Steinbach PA, Tsien RY
  TITLE     Evolution of new nonantibody proteins via iterative somatic 
            hypermutation.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16745-9. Epub 2004 Nov
            19.
  PUBMED    15556995
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2004-11-30"; volume: "101(48)"; pages: 
            "16745-9. Epub 2004 Nov 19"
COMMENT     Sequence Label: mPlum-pBAD
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..74
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..147
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             160..180
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     588..607
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
       61 ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca tggtatggct
      121 agcatgactg gtggacagca aatgggtcgg gatctgtacg agaacctgta cttccagggc
      181 tcgagcatgg tgagcaaggg cgaggaggtc atcaaggagt tcatgcgctt caaggagcac
      241 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      301 tacgagggca cccagaccgc caggctgaag gtgaccaagg gtggccccct gcccttcgcc
      361 tgggacatcc tgtcccctca gatcatgtac ggctccaagg cctacgtgaa gcaccccgcc
      421 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      481 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
      541 ttcatctaca aggtgaaggt gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
      601 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
      661 aagggcgaga tgaagatgag gctgaggctg aaggacggcg gccactacga cgccgaggtc
      721 aagaccacct acatggccaa gaagcccgtg cagctgcccg gcgcctacaa gaccgacatc
      781 aagctggaca tcacctcc
//
