LOCUS       sequence-93366-e        5647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses lncRNA MEG3 isoform c in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Zhou Y, Zhong Y, Wang Y, Zhang X, Batista DL, Gejman R, Ansell PJ, 
            Zhao J, Weng C, Klibanski A
  TITLE     Activation of p53 by MEG3 non-coding RNA.
  JOURNAL   J Biol Chem. 2007 Aug 24;282(34):24731-42. Epub 2007 Jun 13.
  PUBMED    17569660
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-24"; volume: "282(34)"; pages: "24731-42. 
            Epub 2007 Jun 13"
COMMENT     Sequence Label: pCI-MEG3c
FEATURES             Location/Qualifiers
     source          1..5647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2761..2882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2772..2791)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2826..2845
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3063..3518
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3150..3169)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3360..3381
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3585..3604)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3704..3726
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3764..3782)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3850..3954
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3955..4023,4024..4815)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4173..4192)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4986..5574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5475..5494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattcgc agagagggag
     1081 cgcgccttgg ctcgctggcc ttggcggcgg ctcctcagga gagctggggc gcccacgaga
     1141 ggatccctca cccgggtctc tcctcaggga tgacatcatc cgtccacctc cttgtcttca
     1201 aggaccacct cctctccatg ctgagctgct gccaaggggc ctgctgccca tctacacctc
     1261 acgagggcac taggagcacg gtttcctgga tcccaccaac atacaaagca gccactcact
     1321 gacccccagg accaggatgg caaaggatga agaggaccgg aactgaccag ccagctgtcc
     1381 ctcttaccta aagacttaaa ccaatgccct agtgaggggg cattgggcat taagccctga
     1441 cctttgctat gctcatactt tgactctatg agtactttcc tataagtctt tgcttgtgtt
     1501 cacctgctag caaactggag tgtttccctc cccaaggggg tgtcagtctt tgtcgactga
     1561 ctctgtcatc acccttatga tgtcctgaat ggaaggatcc ctttgggaaa ttctcaggag
     1621 ggggacctgg gccaagggct tggccagcat cctgctggca actccaaggc cctgggtggg
     1681 cttctggaat gagcatgcta ctgaatcacc aaaggcacgc ccgacctctc tgaagatctt
     1741 cctatccttt tctgggggaa tggggtcgat gagagcaacc tcctagggtt gttgtgagaa
     1801 ttaaatgaga taaaagaggc ctcaggcagg atctggcata gaggaggtga tcagcaaatg
     1861 tttgttgaaa aggtttgaca ggtcagtccc ttcccacccc tcttgcttgt cttacttgtc
     1921 ttatttattc tccaacagca ctccaggcag cccttgtcca cgggctctcc ttgcatcagc
     1981 aggttcccag tgcccccgac aagcccctgc tggtgtctcc atctcctgcc aagcatcctc
     2041 cagtgcctcc tcctgtgggc ctggcctcag ggctatggac agactcctgt cccatcccag
     2101 agacccctcg tgatcgttcc ctgtagtcac cccactccac cgaaaagtaa actgcaggca
     2161 cgtgggccgt ggcccggctg ggtcggctga agaactgcgg atggaagctg cggaagaggc
     2221 cctgatgggg cccaccatcc cggacccaag tcttcttcct ggcgggcctc tcgtctcctt
     2281 cctggtttgg gcggaagcca tcacctggat gcctacgtgg gaagggacct cgaatgtggg
     2341 accccagccc ctctccagct cgaaatccct ccacagccac ggggacaccc tgcacctatt
     2401 cccacgggac aggctggacc cagagactct ggacccgggg cctccccttg agtagagacc
     2461 cgccctctga ctgatggacg ccgctgacct ggggtcagac ccgtgggctg gacccctgcc
     2521 caccccgcag gaaccctgag gcctagggga gctgttgagc cttcagtgtc tgcatgtggg
     2581 aagtgggctc cttcacctac ctcacagggc tgttgtgagg ggcgctgtga tgcggttcca
     2641 aagcacaggg cttggcgcac cccactgtgc tctcaataaa tgtgtttcct gtcttaacaa
     2701 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagcg gccgcttcga gcagacatga
     2761 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     2821 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2881 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
     2941 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg ggctggcgta
     3001 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     3061 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3121 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3181 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3241 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3301 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3361 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     3421 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3481 taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt
     3541 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     3601 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     3661 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     3721 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     3781 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     3841 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3901 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3961 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4021 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4081 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4141 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4201 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4261 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4321 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4381 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4441 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4501 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4561 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4621 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4681 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4741 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4801 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4861 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4921 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4981 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5041 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5101 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5161 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5221 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5281 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5341 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5401 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5461 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5521 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5581 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga
     5641 cagatct
//
