LOCUS       sequence-93368-e        5593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses lncRNA MEG3 isoform d in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Zhou Y, Zhong Y, Wang Y, Zhang X, Batista DL, Gejman R, Ansell PJ, 
            Zhao J, Weng C, Klibanski A
  TITLE     Activation of p53 by MEG3 non-coding RNA.
  JOURNAL   J Biol Chem. 2007 Aug 24;282(34):24731-42. Epub 2007 Jun 13.
  PUBMED    17569660
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-24"; volume: "282(34)"; pages: "24731-42. 
            Epub 2007 Jun 13"
COMMENT     Sequence Label: pCI-MEG3d
FEATURES             Location/Qualifiers
     source          1..5593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2707..2828
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2718..2737)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2772..2791
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3009..3464
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3096..3115)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3306..3327
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3531..3550)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3650..3672
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3710..3728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3796..3900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3901..3969,3970..4761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4119..4138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4932..5520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5421..5440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattcgg gagcgcgcct
     1081 tggctcgctg gccttggcgg cggctcctca ggagagctgg ggcgcccacg agaggatccc
     1141 tcacccgggt ctctcctcag ggatgacatc atccgtccac ctccttgtct tcaaggacca
     1201 cctcctctcc atgctgagct gctgccaagg ggcctgctgc ccatctacac ctcacgaggg
     1261 cactaggagc acggtttcct ggatcccacc aacatacaaa gcagccactc actgaccccc
     1321 aggaccagga tggcaaagga tgaagaggac cggaactgac cagccagctg tccctcttac
     1381 ctaaagactt aaaccaatgc cctagtgagg gggcattggg cattaagccc tgacctttgc
     1441 tatgctcata ctttgactct atgagtactt tcctataagt ctttgcttgt gttcacctgc
     1501 tagcaaactg gagtgtttcc ctccccaagg gggtgtcagt ctttgtcgac tgactctgtc
     1561 atcaccctta tgatgtcctg aatggaagga tccctttggg aaattctcag gagggggacc
     1621 tgggccaagg gcttggccag catcctgctg gcaactccaa ggccctgggt gggcttctgg
     1681 aatgagcatg ctactgaatc accaaaggca cgcccgacct ctctgaagat cttcctatcc
     1741 ttttctgggg gaatggggtc gatgagagca acctcctagg gttgttgtga gaattaaatg
     1801 agataaaaga ggcctcaggc aggatctggc atagaggagg tgatcagcaa atgtttgttg
     1861 aaaaggtttg acaggtcagt cccttcccac ccctcttgct tgtcttactt gtcttattta
     1921 ttctccaaca gcactccagg cagcccttgt ccacgggctc tccttgcatc aggtaggggc
     1981 tttgcagaga ccatcgacgg gctgacttga ccaagcttct tgaaaggcct gtctacactt
     2041 gctgtcttcc ttcctcacct ccaatttcct cttcaaccca ctgcttcctg actcgctcta
     2101 ctccgtggaa gcacgctcac aaaggcacgt gggccgtggc ccggctgggt cggctgaaga
     2161 actgcggatg gaagctgcgg aagaggccct gatggggccc accatcccgg acccaagtct
     2221 tcttcctggc gggcctctcg tctccttcct ggtttgggcg gaagccatca cctggatgcc
     2281 tacgtgggaa gggacctcga atgtgggacc ccagcccctc tccagctcga aatccctcca
     2341 cagccacggg gacaccctgc acctattccc acgggacagg ctggacccag agactctgga
     2401 cccggggcct ccccttgagt agagacccgc cctctgactg atggacgccg ctgacctggg
     2461 gtcagacccg tgggctggac ccctgcccac cccgcaggaa ccctgaggcc taggggagct
     2521 gttgagcctt cagtgtctgc atgtgggaag tgggctcctt cacctacctc acagggctgt
     2581 tgtgaggggc gctgtgatgc ggttccaaag cacagggctt ggcgcacccc actgtgctct
     2641 caataaatgt gtttcctgtc ttaacaaaaa aaaaaaaaaa aaagcggccg cttcgagcag
     2701 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     2761 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     2821 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     2881 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgggct
     2941 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     3001 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3061 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3121 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3181 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3241 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3301 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     3361 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     3421 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
     3481 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     3541 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     3601 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     3661 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     3721 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3781 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3841 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3901 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3961 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4021 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4081 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4141 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4201 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4261 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4321 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4381 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4441 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4501 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4561 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4621 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4681 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4741 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4801 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4861 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4921 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4981 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5041 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5101 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5161 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5221 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5281 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5341 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5401 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5461 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5521 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5581 gctcgacaga tct
//
