LOCUS       sequence-93377-e         617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN designed to target the human AAVS1/PPP1R12C locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D
  TITLE     An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome 
            Editing in Human Pluripotent Stem Cells.
  JOURNAL   Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018.
  PUBMED    24931489
REFERENCE   2  (bases 1 to 617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018."
COMMENT     Sequence Label: AAVS1-TALEN-R
FEATURES             Location/Qualifiers
     source          1..617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..586)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEIN"
ORIGIN
        1 aattgatctc gccattgtta aacttgcgtc tgacttcttc cagtgtcaga gttcccgctt
       61 tgatcatttc tccaccaatg agcagttcct ctacggacaa aaccgcacca ttgcagttcg
      121 taatgtgatt caaccgtgtg agctgggcct tatagttgcc tttgaaatgc ccagagacaa
      181 acaaaaactt aaactcggtc actgatgaag gatacacttt ccaccactca tttggattga
      241 tgtgtttgtt cctggtctga ttttcttcga cataacgttg catctcatct gcctgcccga
      301 tgggaagatt gtacccaccg ctgtaggctt tcgtgtcgac gatcactccg taatcaatcg
      361 ggctccccac cgtatagatt gctccgtcgg gttttcgtga tccaccgaga tgcttccctc
      421 ggtatccata aaccttcata aagaactcca ttaccttcat ctcaaggatt ctgtcttgag
      481 tcgagttcct agcaatttcg ataagttcga tgtattcatg cggtacgtat ttcaatttgt
      541 gccgcagctc cgactttttc tcctcaagtt cactcttcac gagttgggaa cctgccactc
      601 gatgtgatgt cctctcg
//
