LOCUS       sequence-93378-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  noninfectious (non-packaging), self-replicating Venezuelan equine 
            encephalitis (VEE) virus RNA replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: SP6-VEE-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcgagct
       61 cgatttaggt gacactatag atgggcggcg catgagagaa gcccagacca attacctacc
      121 caaaatggag aaagttcacg ttgacatcga ggaagacagc ccattcctca gagctttgca
      181 gcggagcttc ccgcagtttg aggtagaagc caagcaggtc actgataatg accatgctaa
      241 tgccagagcg ttttcgcatc tggcttcaaa actgatcgaa acggaggtgg acccatccga
      301 cacgatcctt gacattggaa gtgcgcccgc ccgcagaatg tattctaagc acaagtatca
      361 ttgtatctgt ccgatgagat gtgcggaaga tccggacaga ttgtataagt atgcaactaa
      421 gctgaagaaa aactgtaagg aaataactga taaggaattg gacaagaaaa tgaaggagct
      481 ggccgccgtc atgagcgacc ctgacctgga aactgagact atgtgcctcc acgacgacga
      541 gtcgtgtcgc tacgaagggc aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc
      601 gacaagtctc tatcaccaag ccaataaggg agttagagtc gcctactgga taggctttga
      661 caccacccct tttatgttta agaacttggc tggagcatat ccatcatact ctaccaactg
      721 ggccgacgaa accgtgttaa cggctcgtaa cataggccta tgcagctctg acgttatgga
      781 gcggtcacgt agagggatgt ccattcttag aaagaagtat ttgaaa
//
