LOCUS       sequence-93379-e         486 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent membrane voltage sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Zou P, Zhao Y, Douglass AD, Hochbaum DR, Brinks D, Werley CA, 
            Harrison DJ, Campbell RE, Cohen AE
  TITLE     Bright and fast multicoloured voltage reporters via electrochromic 
            FRET.
  JOURNAL   Nat Commun. 2014 Aug 13;5:4625. doi: 10.1038/ncomms5625.
  PUBMED    25118186
REFERENCE   2  (bases 1 to 486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-13"; volume: "5:4625. doi"; pages: " 
            10.1038/ncomms5625"
COMMENT     Sequence Label: FCK-QuasAr2-Citrine
FEATURES             Location/Qualifiers
     source          1..486
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(135..386)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(177..198)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattctta tacttcattc
       61 tcataacaaa atccacctac atttatgtct atttgatcca gggggatgta ctcgccttcg
      121 cttgtgattc tactcttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      181 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
      241 tagctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      301 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
      361 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
      421 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      481 atgcgg
//
