LOCUS       sequence-93386-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN designed to target the human AAVS1/PPP1R12C locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D
  TITLE     An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome 
            Editing in Human Pluripotent Stem Cells.
  JOURNAL   Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018.
  PUBMED    24931489
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018."
COMMENT     Sequence Label: AAVS1-TALEN-R
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    258..274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(730..747)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(730..746)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
       61 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
      121 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
      181 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt caacgccact
      241 gtgcatgaag ctcgtaattg ttatccgctc acaattaagg gcgacacaaa atttattcta
      301 aatgataata aatactgata acatcttata gtttgtatta tattttgtat tatcgttgac
      361 atgtataatt ttgatatcaa aaactgattt tccctttatt attttcgaga tttattttct
      421 taattctctt taacaaacta gaaatattgt atatacaaaa aatcataaat aatagatgaa
      481 tagtttaatt ataggtgttc atcaatcgaa aaagcaacgt atcttattta aagtgcgttg
      541 cttttttctc atttataagg ttaaataatt ctcatatatc aagcaaagtg acaggcgccc
      601 ttaaatattc tgacaaatgc tctttcccta aactcccccc ataaaaaaac ccgccgaagc
      661 gggtttttac gttatttgcg gattaacgat tactcgttat cagaaccgcc cagggggccc
      721 gagcttaaga ctggccgtcg ttttacaaca cagaaagagt ttgtagaaac
//
