LOCUS       sequence-9339-ex        5996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Mentis GZ, Gravell M, Hamilton R, Shneider NA, O'Donovan MJ, 
            Schubert M
  TITLE     Transduction of motor neurons and muscle fibers by intramuscular 
            injection of HIV-1-based vectors pseudotyped with select rabies 
            virus glycoproteins.
  JOURNAL   J Neurosci Methods. 2006 Oct 30. 157(2):208-17.
  PUBMED    16725205
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods. 2006 Oct 30. 157(2):208-17."
COMMENT     Sequence Label: pMD.RVG.CVS24-B2c
FEATURES             Location/Qualifiers
     source          1..5996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        463..666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     617..637
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     663..687
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          800..1275
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     polyA_signal    3002..3396
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(3728..3746)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3814..3918
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4103..4482
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(4137..4156)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     5316..5335
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
       61 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      721 catagaagac accgggaccg atccagcctc ccctcgaagc ttacatgtgg taccgagctc
      781 ggatcctgag aacttcaggg tgagtctatg ggacccttga tgttttcttt ccccttcttt
      841 tctatggtta agttcatgtc ataggaaggg gagaagtaac agggtacaca tattgaccaa
      901 atcagggtaa ttttgcattt gtaattttaa aaaatgcttt cttcttttaa tatacttttt
      961 tgtttatctt atttctaata ctttccctaa tctctttctt tcagggcaat aatgatacaa
     1021 tgtatcatgc ctctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca
     1081 atagcaatat ttctgcatat aaatatttct gcatataaat tgtaactgat gtaagaggtt
     1141 tcatattgct aatagcagct acaatccagc taccattctg cttttatttt atggttggga
     1201 taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt catacctctt
     1261 atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca tcactttggc
     1321 aaagaattcg gcgcgccaag gaaagatggt tcctcaggtt cttttgtttg tactccttct
     1381 gggtttttcg ttgtgtttcg ggaagttccc catttacacg ataccagaca aacttggtcc
     1441 ctggagccct attgacatac accatctccg ctgtccaaat aacctggttg tggaggatga
     1501 aggatgtatc aacctgtccg ggttctccta catggaactc aaagtgggat acatctcagc
     1561 catcaaagtg aacgggttca cttgcacagg tgttgtgaca gaggcagaga cctacaccaa
     1621 ctttgttggt tatgtcacaa ccacattcaa gagaaagcat ttccgcccca ccccagacgc
     1681 atgtagagcc gcgtataact ggaagatggc cggtgacccc agatatgaag agtccctaca
     1741 aaatccatac cccgactacc actggcttcg aactgtaaga accaccaaag agtccctcat
     1801 tatcatatcc ccaagtgtga cagatttgga cccatatgac aaatcccttc actcaagggt
     1861 cttccctggc ggaaagtgct caggaataac ggtgtcctct acctactgct caactaacca
     1921 tgattacacc atttggatgc ccgagaatcc gagaccaggg acaccttgtg acatttttac
     1981 caatagcaga gggaagagag catccaacgg gaacaagact tgcggctttg tggatgaaag
     2041 aggcctgtat aagtctctaa aaggagcatg caggctcaag ttatgtggag ttcttggact
     2101 tagacttatg gatggaacat gggtcgcgat gcaaacatca gatgagacca aatggtgctc
     2161 tccagatcag ttggtgaatt tgcacgactt tcgctcagac gagattgagc atctcgttgt
     2221 ggaggagtta gtcaagaaaa gagaggaatg tctggataca ttagagtcca tcatgaccac
     2281 caagtcagta agtttcagac gtctcagtca cctgagaaaa cttgtcccag ggtttggaaa
     2341 agcatatacc atattcaaca aaaccttgat ggaggctgat gctcactaca agtcagtccg
     2401 gacctggaat gagatcatcc cctcaaaagg gtgtttgaaa gttggaggaa ggtgccatcc
     2461 tcatgtgaac ggggtgtttt tcaatggtat aatattaggg cctgacgacc gtgtcctaat
     2521 cccagagatg caatcatccc tcctccggca acatatggag ttgttggaat cttcagttat
     2581 ccccctgatg caccccctgg ctgacccttc tacagttttc aaagaaggtg atgaggctga
     2641 ggattttgtt gaagttcacc tccccgatgt gtacaaacag atctcagggg ttgacctggg
     2701 tctcccgaac tggggaaagt atgtattgat gactgcaggg gccatgattg gcctggtgtt
     2761 gatattttcc ctaatgacat ggtgcagaag agccaatcga ccagaatcga aacaacgcag
     2821 ttttggaggg acagggggga atgtgtcagt cacttcccaa agcggaaaag tcataccttc
     2881 atgggaatca tataagagtg gaggtgagat cagactgtga cgtacgaccc caccagtgca
     2941 ggctgcctat cagaaagtgg tggctggtgt ggctaatgcc ctggcccaca agtatcacta
     3001 agctcgcttt cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta
     3061 ctaaactggg ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat
     3121 ttattttcat tgcaatgatg tatttaaatt atttctgaat attttactaa aaagggaatg
     3181 tgggaggtca gtgcatttaa aacataaaga aatgaagagc tagttcaaac cttgggaaaa
     3241 tacactatat cttaaactcc atgaaagaag gtgaggctgc aaacagctaa tgcacattgg
     3301 caacagcccc tgatgcctat gccttattca tccctcagaa aaggattcaa gtagaggctt
     3361 gatttggagg ttaaagtttt gctatgctgt attttacatt acttattgtt ttagctgtcc
     3421 tcatgaatgt cttttcacta cccatttgct tatcctgcat ctctcagcct tgactccact
     3481 cagttctctt gcttagagat accacctttc ccctgaagtg ttccttccat gttttacggc
     3541 gagatggttt ctcctcgcct ggccactcag ccttagttgt ctctgttgtc ttatagaggt
     3601 ctacttgaag aaggaaaaac agggggcatg gtttgactgt cctgtgagcc cttcttccct
     3661 gcctccccca ctcacagtga cccggaatcc ctcgacatgg cagtctagaa ttcttgaaga
     3721 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     3781 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3841 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3901 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3961 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4021 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4081 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4141 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     4201 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4261 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4321 ttacttctga caacgatcgg aggaccgaag gagctaaccc gcttttttgc acaacatggg
     4381 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4441 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4501 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4561 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4621 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4681 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4741 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4801 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggctgctgc
     4861 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4921 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     4981 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5041 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5101 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5161 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5221 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5281 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5341 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5401 cggagcctat ggaaaaacgc cagcaacgga gatgcgccgc gtgcggctgc tggagatggc
     5461 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
     5521 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt
     5581 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
     5641 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc
     5701 gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct
     5761 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
     5821 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
     5881 ggggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     5941 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccat
//
