LOCUS       sequence-93396-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses B18R protein from Western vaccinia virus that binds to and
            neutralizes type I IFNs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: pTNT-B18R
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        136..154
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        160..178
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     160..177
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      214..223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
       61 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggct cgacagatct
      121 taaggctaga gtacttaata cgactcacta taggctagca tttaggtgac actatagaat
      181 acaagctact tgttcttttt gcactcgaga attgccacca tgggcacgat gaaaatgatg
      241 gtacatatat atttcgtatc attattgtta ttgctattcc acagttacgc catagacatc
      301 gaaaatgaaa tcacagaatt cttcaataaa atgagagata ctctaccagc taaagactct
      361 aaatggttga atccagcatg tatgttcgga ggcacaatga atgatatagc cgctctagga
      421 gagccattca gcgcaaagtg tcctcctatt gaagacagtc ttttatcgca cagatataaa
      481 gactatgtgg ttaaatggga aaggctagaa aaaaatagac ggcgacaggt ttctaataaa
      541 cgtgttaaac atggtgattt atggatagcc aactatacat ctaaattcag taaccgtagg
      601 tatttgtgca ccgtaactac aaagaatggt gactgtgttc agggtatagt tagatctcat
      661 attagaaaac ctccttcatg cattccaaaa acatatgaac taggtactca tgataagtat
      721 ggcatagact tatactgtgg aattctttac gcaaaacatt ataataatat aacttggtat
      781 aaagataata aggaaattaa tatcgacgac attaagtatt cacaaacggg aaaggaatta
      841 attattcata atccagagtt agaagatagc ggaagatacg actgttacgt tcattacgac
//
