LOCUS       sequence-93404-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: SP6-VEE-OKS-iM
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atttctactg cgacgaggag gagaacttct accagcagca gcagcagagc gagctgcagc
       61 ccccggcgcc cagcgaggat atctggaaga aattcgagct gctgcccacc ccgcccctgt
      121 cccctagccg ccgctccggg ctctgctcgc cctcctacgt tgcggtcaca cccttctccc
      181 ttcggggaga caacgacggc ggtggcggga gcttctccac ggccgaccag ctggagatgg
      241 tgaccgagct gctgggagga gacatggtga accagagttt catctgcgac ccggacgacg
      301 agaccttcat caaaaacatc atcatccagg actgtatgtg gagcggcttc tcggccgccg
      361 ccaagctcgt ctcagagaag ctggcctcct accaggctgc gcgcaaagac agcggcagcc
      421 cgaaccccgc ccgcggccac agcgtctgct ccacctccag cttgtacctg caggatctga
      481 gcgccgccgc ctcagagtgc atcgacccct cggtggtctt cccctaccct ctcaacgaca
      541 gcagctcgcc caagtcctgc gcctcgcaag actccagcgc cttctctccg tcctcggatt
      601 ctctgctctc ctcgacggag tcctccccgc agggcagccc cgagcccctg gtgctccatg
      661 aggagacacc gcccaccacc agcagcgact ctgaggagga acaagaagat gaggaagaaa
      721 tcgatgttgt ttctgtggaa aagaggcagg ctcctggcaa aaggtcagag tctggatcac
      781 cttctgctgg aggccaca
//
