LOCUS       sequence-93409-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 568, Emission:
            592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mApple-pBAD
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..729)
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(297..316)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(join(745..759,760..768))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 cagccaagct tcgaattctt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc
       61 ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggaca cgatgtccaa
      121 cttgatgtcg acgatgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt
      181 cttgacctcg gcggcgtagt ggccgccgtc cttcagcttc agcctcttct tgatctcgct
      241 cttcagggcg ccgtcctcgg ggtacatccg ctcctcggag gcctcccagc ccatggtctt
      301 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
      361 gaacacgccg tcctgcaggg aggagtcctg gttaacgtga ataatgccgc cgtcctcgaa
      421 gttcatcacg cgctcccacc tgaagccctc ggggaaggac agcttgaagt agtcggggat
      481 gtcggctggg tgcttaatgt agaccttgga gccgtacatg aactgagggg acaggatgtc
      541 ccaggcgaag ggcagggggc cacccttggt caccttcagc ttagcggtct gaaaggcctc
      601 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
      661 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttattct cctcgccctt
      721 gctcaccatg ctcgagctcg gatccttatc gtcatcgtcg tacagatccc g
//
