LOCUS       sequence-93412-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 383, Emission:
            448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: EBFP2-pBAD
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(13..726,727..729,730..732))
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     666..687
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agcttcgaat tcttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtgcg
       61 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gcacggactg
      121 ggtgctcagg tagtggctgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      181 ctggtagtgg tcggcgagct gcacgctgcc gtcctccacg ttgtggcgga tcttgaagtt
      241 caccttgatg ccgttcttct gcttgacggc catgatatag atgttgtggc tgttgaagtt
      301 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgacgc ccttcagctc
      361 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtaggtgcc
      421 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
      481 gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag cactgcacgc cgtggctcag
      541 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
      601 ggtcagcttg ccgttggtgg catcgccctc gccctcgccc ctcacgctga acttgtggcc
      661 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
      721 cttgctcacc atgctcgagc cctgg
//
