LOCUS       sequence-93413-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondrial Zn-binding FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Park JG, Qin Y, Galati DF, Palmer AE
  TITLE     New Sensors for Quantitative Measurement of Mitochondrial Zn(2+).
  JOURNAL   ACS Chem Biol. 2012 Aug 10.
  PUBMED    22850482
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2012 Aug 10."
COMMENT     Sequence Label: pcDNA-mito-ZapCY1
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     80..101
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(378..399)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     727..746
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg
       61 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
      121 aacagctcct cgcccttgct caccatgagc tcgataccat gttgacaatt aatatggttc
      181 actatggaac atgtatcatc tccaaggaaa tcacagtcct cccaattaca agctaatggt
      241 tccatagggt gtttgaaatc ttgagagaca tgatccttca aaagatgtct ttgtaggtca
      301 aatagtgagc tacaagactc aggacattct ttccatttgc attttaagtc attgttttta
      361 tgcatgcggg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      421 tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
      481 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
      541 ttgtggcgga tcttgaagtg ggccttgatg ccgttcttct gcttgtcggc ggtgatatag
      601 acgttgtggc tgatgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      661 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      721 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tacgctcctg gacgtagcct
      781 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtc
//
