LOCUS       sequence-93414-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein,
            flanked by ITRs for AAV packaging. Combine with N-Terminal Split 
            Cas9 Gyra Intein for full length SpCas9 production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G
  TITLE     Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human 
            cells using compact expression cassettes.
  JOURNAL   Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777.
  PUBMED    26126518
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-07-1"; volume: "5:10777. doi"; pages: " 
            10.1038/srep10777"
COMMENT     Sequence Label: C-Terminal Split Cas9 with GyrA intein
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..58)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ggaattctta ctttttcttt tttgcctggc cggccttttt cgtggccgcc ggccttttgt
       61 cgcctcccag ctgagacagg tcgatccgtg tctcgtacag gccggtgatg ctctggtgga
      121 tcagggtggc gtccagcacc tctttggtgc tggtgtacct cttccggtcg atggtggtgt
      181 caaagtactt gaaggcggca ggggctccca gattggtcag ggtaaacagg tggatgatat
      241 tctcggcctg ctctctgatg ggcttatccc ggtgcttgtt gtaggcggac agcactttgt
      301 ccagattagc gtcggccagg atcactctct tggagaactc gctgatctgc tcgatgatct
      361 cgtccaggta gtgcttgtgc tgttccacaa acagctgttt ctgctcatta tcctcggggg
      421 agcccttcag cttctcatag tggctggcca ggtacaggaa gttcacatat ttggagggca
      481 gggccagttc gtttcccttc tgcagttcgc cggcagaggc cagcattctc ttccggccgt
      541 tttccagctc gaacagggag tacttaggca gcttgatgat caggtccttt ttcacttctt
      601 tgtagccctt ggcttccaga aagtcgatgg gattcttctc gaagctgctt ctttccatga
      661 tggtgatccc cagcagctct ttcacactct tcagtttctt ggacttgccc ttttccactt
      721 tggccaccac cagcacagaa taggccacgg tggggctgtc gaagccgccg tacttcttag
      781 ggtcccagtc cttctttctg gcgatcagct tatcgctgtt cctcttgggc aggatagact
      841 ctttgctgaa gccgcctgtc tgcacctcgg tctttttcac gatattcact tggggcatgc
      901 tcagcacttt ccgcacggtg gcaaaatccc ggcccttatc ccacacgatc tccccggttt
      961 cgccgtttgt ctcgatcaga ggccg
//
