LOCUS       sequence-93416-e        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein,
            flanked by ITRs for AAV packaging. Combine with N-Terminal Split 
            Cas9 Gyra Intein for full length SpCas9 production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G
  TITLE     Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human 
            cells using compact expression cassettes.
  JOURNAL   Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777.
  PUBMED    26126518
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-07-1"; volume: "5:10777. doi"; pages: " 
            10.1038/srep10777"
COMMENT     Sequence Label: C-Terminal Split Cas9 with GyrA intein
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggccttatcc agttcgctca ggccgcctct ctcggccttg gtcagattgt cgaactttct
       61 ctgggtaatc agcttggcgt tcagcagctg ccgccagtag ttcttcatct tcttcacgac
      121 ctcttcggag ggcacgttgt cgctcttgcc ccggttcttg tcgcttctgg tcagcacctt
      181 gttgtcgatg gagtcgtcct tcagaaagct ctgaggcacg atatggtcca catcgtagtc
      241 ggacagccgg ttgatgtcca gttcctggtc cacgtacata tcccgcccat tctgcaggta
      301 gtacaggtac agcttctcgt tctgcagctg ggtgttttcc acggggtgtt ctttcaggat
      361 ctggctgccc agctctttga tgccctcttc gatccgcttc attctctcgc ggctgttctt
      421 ctgtcccttc tgggtggtct ggttctctct ggccatttcg atcacgatgt tctcgggctt
      481 gtgccggccc atcactttca cgagctcgtc caccaccttc actgtctgca ggatgccctt
      541 cttaatggcg gggctgccgg ccagattggc aatgtgctcg tgcaggctat cgccctggcc
      601 ggacacctgg gctttctgga tgtcctcttt aaaggtcagg ctgtcgtcgt ggatcagctg
      661 catgaagttt ctgttggcga agccgtcgga cttcaggaaa tccaggattg tcttgccgga
      721 ctgcttgtcc cggatgccgt tgatcagctt ccggctcagc ctgccccagc cggtattgtg
      781 actaacgaag ccattggtaa taaaagcatg gtcggctgtg tccaccctaa gagaatacac
      841 aggctgtacg cctgcatcgg tcacagaggc gaccttagca taataaaacc tcccgtcggt
      901 cagctcgtct gcgattgcct gagcgtctgg atccctgtgg tgggcttcca ggaatcgaac
      961 cagccctggg acgccgacag tatatgtagt tggggcaaat tccggttttc c
//
