LOCUS       sequence-93417-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondrial Zn-binding FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Park JG, Qin Y, Galati DF, Palmer AE
  TITLE     New Sensors for Quantitative Measurement of Mitochondrial Zn(2+).
  JOURNAL   ACS Chem Biol. 2012 Aug 10.
  PUBMED    22850482
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2012 Aug 10."
COMMENT     Sequence Label: pcDNA-mito-ZifCV1.173
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             65..139
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             161..235
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             257..331
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     complement(407..428)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(668..687)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cttgacaggc tcggcccggc ggctcccagt gccgcgcgcc aagatccatt cgttgggaga
       61 tcctatgtcc gtcctgacgc cgctgctgct gcggggcttg acaggctcgg cccggcggct
      121 cccagtgccg cgcgccaaga tccattcgtt gggagatcct atgtccgtcc tgacgccgct
      181 gctgctgcgg ggcttgacag gctcggcccg gcggctccca gtgccgcgcg ccaagatcca
      241 ttcgttggga gatcctatgt ccgtcctgac gccgctgctg ctgcggggct tgacaggctc
      301 ggcccggcgg ctcccagtgc cgcgcgccaa gatccattcg ttggggaagc ttggggatcc
      361 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      421 cggcgacgta aacggccaca ggttcagcgt gtccggcgag ggcgagggcg atgccaccta
      481 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      541 cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
      601 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gtaccatctt
      661 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      721 ggtgaaccgc atcgagctga agggcatcga cttc
//
