LOCUS       sequence-93420-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein,
            flanked by ITRs for AAV packaging. Combine with C-Terminal Split 
            Cas9 Gyra Intein for full length SpCas9 production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G
  TITLE     Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human 
            cells using compact expression cassettes.
  JOURNAL   Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777.
  PUBMED    26126518
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-07-1"; volume: "5:10777. doi"; pages: " 
            10.1038/srep10777"
COMMENT     Sequence Label: N-Terminal Split Cas9 with GyrA intein
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gaattcttag cgagcgaagc cagcgcaatc cacactaaat gcggaccttt ggatcactgc
       61 atagtctccg ggttttatct catcaatcag tttccacagc agggtgggga cgccggcaac
      121 atccaccaga cacaaaaggg ggtggttggc tgtgcctgtc actctcaggc cttctacggt
      181 gcggacggta tacacaggat gttcaccact gtggaagagt ctatcggcca gaacaggatt
      241 gccgtggcgg tcaagcacct tcaggtcgat ggcgttatcg ctattagggc gggcgcccgg
      301 gacgatgtca gcgatgcgca ctgattcgcc ttcgggcagc gccaccagag catcaccggt
      361 gatacagtat ctccgccgct tcagctgctt catcactttg tcgtcgaaca ggtgggcata
      421 ggttttcagc cgttcctcga tcatctctct gtcctcaaac agtgtcaggg tcagcacgat
      481 atcttccaga atgtcctcgt tttcctcatt gtccaggaag tccttgtcct tgataatttt
      541 cagcagatcg tggtatgtgc ccagggaggc gttgaaccga tcttccacgc cggagatttc
      601 cacggagtcg aagcactcga ttttcttgaa gtagtcctct ttcagctgct tcacggtcac
      661 tttccggttg gtcttgaaca gcaggtccac gatggccttt ttctgctcgc cgctcaggaa
      721 ggcgggcttt ctcattccct cggtcacgta tttcactttg gtcagctcgt tatacacggt
      781 gaagtactcg tacagcaggc tgtgcttggg cagcaccttc tcgttgggca ggttcttatc
      841 gaagttggtc atccgctcga tgaagctctg ggcggaagcg cccttgtcca ccacttcctc
      901 gaagttccag ggggtgatgg tttcctcgct ctttctggtc atccaggcga atctgctgtt
      961 tcccctggcc agagggccca cgtagtaggg gatgcggaag gtcaggatct tctcgatctt
     1021 ttcccggttg tccttcagga atgggtaaaa
//
